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2017;114:E3110-8 National Academy of Sciences

Rapid weight loss, hormonal shifts, and medication changes can make this existing tendency more obvious

Hydrogen peroxide solution (H 2 O 2 3%) was added to the slides to prevent endogenous peroxidase activity, followed by primary antibodies (anti-BAX 1:100) (ABclonal , USA) and (anti-GFAP 1:100) (Diagnostic BioSystems, USA) to be incubated with the slides overnight